green fluorescent protein gfp project

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Given:

Below are THREE files attached, The first file is the 14-page project ROUGH DRAFT file that I have worked on for 6 weeks concerning the development, purification, and transformation of the Green protein (GFP) that is synthesized from Bacterium Escherichia Coli

and the second file the first file that is graded by my professor annotated with notes and heads up about everything I messed (Vague info, punctuation mistakes, spelling errors, etc …)

and the third file is the graded rubric (16/40)

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What needs to be done:

Please go over the whole project and correct anything that needs to be corrected, add any info that needs to be added, remove the gray spots from the Vague phrases (make it worth calling a FINAL DRAFT)

my instructor is very picky and she checks for every single detail, so please perform the corrections to the best of your knowledge.

The project is worth 3-exam grades, so my life basically depends on this project!!!

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note:

everything Bio-related term or info is correctly placed but may not be correctly stated.

any additional information or data you will need I will provide in separate files I will attach along in case if need (data files and overall idea about the project)

please take care of this project as if you were doing it for yourself.

thank you

 
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phy2252 its me i forgot my password can you see that

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If you double the load resistance connected to a battery of fixed emf and internal resistance, the current drawn from the battery will necessarily…







Question 25 / 5 pts

If you double the load resistance connected to a battery of fixed emf and internal resistance, the terminal voltage will necessarily…










Question 35 / 5 pts

If you double both the resistance and the capacitance of a certain RC circuit, then the circuit’s half-life will be…









 
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event article

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This application assignment requires you to share a business related article from the past 6 months that describes your view of our assigned course topic(s) for the week. In other words, your view may explain the topic’s effect, relevance, importance, non-significance, etc. in workplace behavior. This assignment should consist of three distinct paragraphs. In the first paragraph you will summarize the article you chose. In the second paragraph you will clearly identify and discuss the course topic(s) relevant to the article. In the third and final paragraph you should discuss your views about the topic and what the article stated.

It is imperative to choose a concept being covered in this module. It is also important not to copy material from other related assignments or directly from the article itself–use proper citations and rewrite important components into your own words. Concepts and verbatim material will receive a zero.

Current Event:

  • News story must be from a credible source within the last 6 months with appropriate citation.
  • Context must be provided on why the story is immediately relevant to the topics being covered.
  • Responses which are purely opinion and anecdotal are not considered to be substantive in nature.
  • Original news story from an external source plus one additional external source required

****Only 3 paragraphs needed. Any current event article can be used *****

Must be APA format, References as well

 
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answer the following questions 424

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Read the following papers:

  1. Brenner, Susan W., and Bert-Jaap Koops. “Approaches to cybercrime jurisdiction.” J. High Tech. L. 4 (2004): 1. (available at: https://www.researchgate.net/profile/Susan_Brenner…
  2. Reidenberg, Joel R., et al. “Internet Jurisdiction: A survey of legal scholarship published in English and United States Case Law.” (2013). (available at: http://scholarship.law.upenn.edu/cgi/viewcontent.cgi?article=1347&context=penn_law_review&sei-redir=1)
  3. California Cable Laws. Available at: https://www.calcable.org/government-relations/stat…

Answer the following questions:

  1. Currently, there are questions over whether it is permissible for ISP’s (major U.S. providers include Comcast, Verizon and AT & T) to control aspects of speed and delivery of content on their netowrks to their subscribers. Many subscribers and sites have complained about these practices, asserting that the ISPs should handle all content the same. What organization or governmental entity should handle this? How should it be resolved?
  2. Discuss the impact of California Laws over ISP traffic content regarding the above cases.
  3. Discuss the approaches to Internet jurisdiction based on laws of different countries
 
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assignment due monday morning 6

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There is more to CAM than many think, and it actually works in conjunction with conventional medicine more than is widely acknowledged. There are CAM therapies available for ailments that many believe only conventional medicine can heal.

Write a 950-word paper that describes an overview of CAM and its categories of treatments. Ensure you:

  • Compare and contrast current conventional medicine regulations and oversights with regulation and oversight of alternative medicine in the United States.
  • Define the terms alternative, complementary, and integrative in relation to medicine and discuss how these terms differ from one another.
  • Describe how conventional medicine plays a role in alternative, complementary, and integrative medicine.
  • Discuss the philosophy of CAM and how it is similar to or different from the approach of conventional Western medicine.
  • Select one CAM therapy from among the alternative medicine approaches and describe how it can be used to treat a medical condition you choose. Include discussion of how your chosen therapy can be used as an alternative, a complementary, or an integrative therapy for that specific medical condition.
  • Include the results of at least one scientific study that show a clinical benefit from the therapy you selected.

Use at least one reference in addition to NCCIH.

 
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information governance 109

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APA format is required for both in-line references and endnote references.

Full-text PDFs of all references are required. Lack of full-text PDFs will lose half of the credit for this post.

Chapter 13 – According to Franks and Smallwood (2013), information has become the lifeblood of every business organization, and that an increasing volume of information today has increased and exchanged through the use of social networks and Web2.0 tools like blogs, microblogs, and wikis. When looking at social media in the enterprise, there is a notable difference in functionality between e-mail and social media, and has been documented by research – “…that social media differ greatly from e-mail use due to its maturity and stability.” (Franks & Smallwood, 2013).

Q1: Please identify and clearly state what the difference is?

Create a new thread with 700 words, be clear and concise!

Textbook: https://b-ok.org/book/2363786/2f8186

I need atleast 2 references and also pdf attachments of the documents used as references, please use atleast 2 references and also refer to text book..and once you complete send me the pdf files of the references and also the cite the references in the discussion.

Textbook Link: Information Governance: Concepts, Strategies and Best Practices; 1st Edition; Robert F. Smallwood; Copyright © 2014 by John Wiley & Sons, Inc., Hoboken, New Jersey (ISBN 978-1-118-21830-3)

 
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read one of the two and answer these questions

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To pass this assignment, you need to:

  1. Answer content questions 1-3 listed below
  2. Each essay must be at least 200 words long
  3. Each essay must be written in full sentences with proper grammar and spelling

After watching the assigned show segment for this week and reading the assigned news articles or policy reports, answer the following questions:

  1. What are the key ideas of the show segment?
  2. What details and/or storytelling devices does the host use to convey the setting, storyline, or broader themes of the show segment?
  3. How comprehensive and accurate is the information portrayed in the video clip compared to the assigned readings? Consider the following questions: Did it exclude information? If so, which one? Will the exclusion impact the political knowledge or awareness of the audience in a negative way? Did it get information wrong? If so, which one? Will the wrong information impact the political knowledge or awareness of the audience in a negative way?

https://www.nytimes.com/interactive/2019/world/asi…

https://www.amnesty.org/en/latest/news/2019/10/tim…

 
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theatre script writing

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Assignment:

In modern television, most series accept script proposals from outside their own hired writers, especially after the series has run a number of years. Your assignment is to choose any television show you would like and write a 500 to 1000 word script for that show. The format can be anything you choose, but needs to make clear who is talking. Any movement or action that is important to the scene but is not apparent from the spoken words needs to be written using parenthesis and italics or a different font from the dialogue font. These descriptions count as part of the word total for your scene.

Example:

John: I can smell you from here.

Sam: (Hiding his cigarette). What do you mean?

Try to have the scene contain a beginning, middle and end. You can assume that the person reading your scene is very well acquainted with the series, as would be the case if you were submitting a speculative script to that show. Have fun. Make something up. Create some new, imagined, direction for the show to take. Please don’t make me search to see if you copied one already done on that show. You never know, my tastes might not be what you think and I might watch the show you are using.

Any type of show is fine. Even reality shows (if you think many of them don’t have scripts, they often do). It does not have to contain all the series’ characters, but the scene needs to mainly be made up from existing characters, with a guest start or two, if you want.

 
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intersectional identity 1

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Explore and present the “intersectional” identity of a catholic middle eastern 21 year old woman that was born in the US. Think of some of the important elements of identity to represent graphically. Think in terms of teaching about her intersectionality and situatedness in society by illustrating who she is.

Choose 5 of the following axes to represent her intersectionality: one of them has to be race or ethnicity

-indigenity, race or ethnicity, social class, gender, and sexuality, religion

make it reflective, explain why those 5 were chosen

clarify how she would interpret values, norms, ideas, and structures that make her identities “real” as social constructions

accurately identify these values, ideas and structures, as well as synthesize across the axes to show how her situatedness in society affects her identity

discuss the axes in relation to each other

identify her position in relation to social normative positions or privileged and oppressed positions for her axes

-discuss her religion as a devout catholic

-discuss her being a part of a low income family, and is the first person in her family to go to college

-discuss the strictness of her immigrant parents (from iraq) and growing up being taught their traditional middle eastern values

have questions? need any more details? let me know 🙂

 
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psy4320 week 2

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Gender Expression & Development

Using the Internet and the South University Online Library, review a variety of media characters, analyzing how male and female characters are portrayed. Media can be videos, cartoons, comic books, movies, etc. What are some common themes and characteristics in these portrayals? What messages do these send to young children about gender roles? Critically evaluate the scientific evidence for gender differences in a variety of areas such as social roles, cognitive abilities, education, self-esteem, and self-confidence.

In addition, address the following:

  • Emotional expression of gender: How did the social cultural aspects of media, observational learning, and other factors influence your perception of gender-appropriate emotional expression?
  • Social expression or expectation of gender: Is there a difference between genders in terms of expression of responsiveness to babies or nurturance? Do we encourage little boys to express feelings aggressively and little girls to articulate and verbalize their emotions?
  • Geographic expression or expectation of gender: Are there differences within or across various cultures and geographical areas?
  • Personality expression of gender: What does personality have to do with expression of emotions?
  • Physiologic expression of gender: How do hormones and physiological changes impact emotions?

Compile your responses in a 2- to 3-page Microsoft Word document. Cite all sources using APA format.

 
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