Hyper-IgE syndrome, also known as Job’s syndrome, is an immunodeficiency disease resulting from the lack of function of a single gene (gene ‘X’). Patients with this disease are highly susceptible to infections with extracellular bacteria and fungi, most frequently including Staphylococcus aureus infections and Candida albicans infections in the skin. Analysis of the various immune cell compartments indicates that these patients have normal numbers of each
cell lineage (i.e., CD4 and CD8 T cells, B cells, monocytes, dendritic cells, NK cells, granulocytes, etc.), and normal levels of IgG, IgA, and IgM antibodies, but higher than normal levels of IgE.
a) Given this information, name a likely component of the immune response that could be impaired in these patients.
To investigate the immune mechanism impaired in these patients, a mouse model of this gene deficiency was generated. Conditional knockout mouse lines were generated in which gene X was knocked out in either the T cells, the B cells, or the myeloid cells of the mouse. For each conditional knockout line, mice were challenged with Candida albicans, and the ability to clear the infection was assessed. In mice, infection of the oral cavity with Candida albicans has been shown to be a valid model for mucosal Candida albicans infections in humans. After infection, the response was assessed by measuring fungal burden (CFU/g tissue) on the tongue. The resulting data are shown in Figure Q6.A.
b) Based on these data, what is the most likely immune function impaired in the Gene Xdeficient patients?
Histological examination of tongue sections from Candida albicans infected mice were examined, and the numbers of infiltrating leukocytes (white blood cells) were quantified in each microscopic field of each section, and the results are shown in Figure Q6.B.
c) Do these data support or refute your hypothesis stated in response to question (b)? Why or why not?
To examine the details of T cell responses when Gene X is absent from the T cells, a series of in vitro experiments were performed. CD4 T cells were isolated from wild type mice and from T cell-deleted Gene X knockout mice, and were stimulated in vitro with a combination of anti-CD3 and anti-CD28 antibodies to activate the T cells. In addition, each culture was supplemented with one of the following cytokine conditions: (1) IFN- plus IL-12; (2) IL-4; or (3) IL-6, TGF-, IL-1 plus IL-23. After four days, the cells were examined for the expression of transcription factors by RT-PCR, as shown in Figure Q6.C. Note that Gene X does not encode T-bet, GATA3, or RORt. Instead, these data indicated impaired responses of Gene X-deficient T cells to the cytokines used in these in vitro culture experiments.
d) Based on these data, name three candidate genes that could be Gene X.
Attached are the figures needed for this question
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Hydrogen Fuel Cells Have Been Considered An Alternative For A Clean Source Of En
/in Uncategorized /by developerHydrogen fuel cells have been considered an alternative for a clean source of energy. In a fuel cell, hydrogen reacts with oxygen to produce water, and at the same time, it releases a great deal of energy. Write an essay that addresses the chemical reaction of hydrogen fuel cells, the energy that is available from this reaction, and whether this type of alternative fuel is feasible. Be complete and use examples to support your statements.
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Hydrogen Gas Is Produced When Zinc Reacts With Sulfuric Acid If 164 Of Wet H2 Is
/in Uncategorized /by developerHydrogen gas is produced when zinc reacts with sulfuric acid: If 164 of wet H2 is collected over water at 24C and a barometric pressure of 739 , how many grams of Zn have been consumed? (The vapor pressure of water is 22.38 .)
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Hydrogen Has Two Naturally Occurring Isotopes 1h And 2h Chlorine Also Has Two Na
/in Uncategorized /by developerHydrogen has two naturally occurring isotopes,1H and 2H. Chlorine also has two naturally occurring isotopes,35Cl and 37Cl. Thus, hydrogen chloride gas consists of four distinct types of molecules: 1H35Cl,1H37Cl,2H35Cl, and2H37Cl. Order these four molecules in order of decreasing rate of effusion.
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Hydrogen Sulfide Ion Hs Is A Weaker Base Than Ammonia Nh3 When Ammonia Reacts W
/in Uncategorized /by developerHydrogen sulfide ion, HS-, is a weaker base than ammonia, NH3. When ammonia reacts with hydrosulfuric acid, H2S, what species are present in the solution in the largest amount?
A. hydrosulfuric acid and ammonia, H2S and NH3
B. hydrosufuric acid and ammonium ion, H2S and NH4+
C. hydrogen sulfide ion and ammonia, HS- and NH3
D. hydrogen sulfide ion and ammonium ion, HS- and NH4+
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Hydrolysis Of Acetyl Phosphate Acetyl P 2 Acetate Pi Has A Standard Free Energy
/in Uncategorized /by developerplease show work and steps!! also show any conversions if any thank u a bunch the answer is given but looking to see the work !
Hydrolysis of acetyl phosphate ( Acetyl -P + 2 acetate + Pi ) has a standard freeenergy change of AG = 42 . 3 KU / mol . What is the free energy change for this reactionwhen the concentrations of acetate , phosphate and acetyl phosphate are 2 mM , 2 mmand 2 n M respectively , and the temperature is 20 C ?a . – 41 6 KJ/ molb . – 430 KJ / molC. – 24. 8 KJ / mold . – 59 . 8 KJ / mole . OKJ /mol
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Hye I Am Nayab And I Just Want To Ask U That I Am Going To Submit My Visa Applic
/in Uncategorized /by developerhye i am NAYAB . and i just want to ask u that i am going to submit my visa application for uk to get visa.a and i am not going to attach original CAS statement with my application. i am attaching my electronic CAS statement which i print out from e mail.is it ok. give me reply as soon as possible
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Hyper Ige Syndrome Also Known As Job S Syndrome Is An Immunodeficiency Disease R
/in Uncategorized /by developerHyper-IgE syndrome, also known as Job’s syndrome, is an immunodeficiency disease resulting from the lack of function of a single gene (gene ‘X’). Patients with this disease are highly susceptible to infections with extracellular bacteria and fungi, most frequently including Staphylococcus aureus infections and Candida albicans infections in the skin. Analysis of the various immune cell compartments indicates that these patients have normal numbers of each
cell lineage (i.e., CD4 and CD8 T cells, B cells, monocytes, dendritic cells, NK cells, granulocytes, etc.), and normal levels of IgG, IgA, and IgM antibodies, but higher than normal levels of IgE.
a) Given this information, name a likely component of the immune response that could be impaired in these patients.
To investigate the immune mechanism impaired in these patients, a mouse model of this gene deficiency was generated. Conditional knockout mouse lines were generated in which gene X was knocked out in either the T cells, the B cells, or the myeloid cells of the mouse. For each conditional knockout line, mice were challenged with Candida albicans, and the ability to clear the infection was assessed. In mice, infection of the oral cavity with Candida albicans has been shown to be a valid model for mucosal Candida albicans infections in humans. After infection, the response was assessed by measuring fungal burden (CFU/g tissue) on the tongue. The resulting data are shown in Figure Q6.A.
b) Based on these data, what is the most likely immune function impaired in the Gene Xdeficient patients?
Histological examination of tongue sections from Candida albicans infected mice were examined, and the numbers of infiltrating leukocytes (white blood cells) were quantified in each microscopic field of each section, and the results are shown in Figure Q6.B.
c) Do these data support or refute your hypothesis stated in response to question (b)? Why or why not?
To examine the details of T cell responses when Gene X is absent from the T cells, a series of in vitro experiments were performed. CD4 T cells were isolated from wild type mice and from T cell-deleted Gene X knockout mice, and were stimulated in vitro with a combination of anti-CD3 and anti-CD28 antibodies to activate the T cells. In addition, each culture was supplemented with one of the following cytokine conditions: (1) IFN- plus IL-12; (2) IL-4; or (3) IL-6, TGF-, IL-1 plus IL-23. After four days, the cells were examined for the expression of transcription factors by RT-PCR, as shown in Figure Q6.C. Note that Gene X does not encode T-bet, GATA3, or RORt. Instead, these data indicated impaired responses of Gene X-deficient T cells to the cytokines used in these in vitro culture experiments.
d) Based on these data, name three candidate genes that could be Gene X.
Attached are the figures needed for this question
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Hypothesis Iii There Is A Positive Relationship Between Myedu Usage And The Ov
/in Uncategorized /by developerI resubmitted! In attached documet, there will be few hypothesis. By using the data I have provided, would you please analyze, interpret, and prove the hypothesis if it is statistically significant or not? It has to be 250-350 words. For example: This research question was answered primariliy through quantitative research. After conducting an XX test, table gives the value as ###(numbers). we found a statistical difference A and B with a significance level of ###. we found a statistical difference A and B with a significance level of ###. A mean satisfaction is higher than B by 0.###, which illustrates that As are more likely to use Course Hero than Bs. Above paragraph is just an example so it is way shorter than it has to be. the answer for the hypothesis needs to be more elaborative since each answer for the hypothesis has to be 250-350 words.
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Hypothesis Iv Females Leave More Feedback And Visit Myedu More Than Males Do I
/in Uncategorized /by developerI revised it into 3 questions so pleasee send me asap! thank you so much!In attached documet, there will be few hypothesis. By using the data I have provided, would you please analyze, interpret, and prove the hypothesis if it is statistically significant or not? It has to be 250-350 words. For example: This research question was answered primariliy through quantitative research. After conducting an XX test, table gives the value as ###(numbers). we found a statistical difference A and B with a significance level of ###. we found a statistical difference A and B with a significance level of ###. A mean satisfaction is higher than B by 0.###, which illustrates that As are more likely to use Course Hero than Bs. Above paragraph is just an example so it is way shorter than it has to be. the answer for the hypothesis needs to be more elaborative since each answer for the hypothesis has to be 250-350 words
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Hypothesis Test For A Population Mean Sigma Known If The Population Standard Dev
/in Uncategorized /by developerScenario 1
Using the Yahoo Finance Web site as in Module 1, download a separate five years of daily data for a stock that begins with the first letter of your first name this time. Be sure that you match the exact dates that you used for the initial stock data, so that you can combine the spreadsheets by pasting.
Now, you are interested in seeing if the mean daily closing volume of the two stocks are statistically different when accounting for daily volatility of the market. In other words, you want to conduct a matched t-test using Date as the variable for pairing. To complete this analysis, do the following:
Scenario 2
Now, use only the original stock data, those data associated with the first letter of your last name. You are interested in whether the mean daily adjusted closing price for the last three years has increased from the mean daily closing price of the first two years. Conduct a hypothesis test by doing the following.
Practical Application Scenario 3
You are interested in knowing whether there are differences in the mean daily adjusted closing price for a specific stock. For this practical application, go back to Yahoo! Finance and download five-years of monthly data for the stock beginning with the first letter of your last name. To do so simply requires you to change the frequency to monthly. Then conduct a test to see whether there are monthly differences in the mean adjusted closing prices.
Running head: COMPARING MULTIPLE SAMPLES Comparing multiple samplesNameInstitution 1 COMPARING MULTIPLE SAMPLESPractical application Scenario 1This scenario involves comparing the mean daily…
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