Hi, need to submit a 2000 words essay on the topic MALDI technique & FLOW CYTOMETRY technique.
(2) ionization of sample components to produce ions, (3) separation of ions by electromagnetic field to their mass-to-charge ratios, (4) detection of ion signals and, (5) processing of the signals into mass spectra.
The ionization of the analytes is a crucial step in mass spectrometry. Two methods are used: electron spray ionization and matrix-assisted laser desorption ionization (MALDI) (Figure 1). In MALDI, the analyte is mixed with matrix, molecules that have strong absorbance at the laser wavelength, and placed onto a metal plate (Karas, Bachmann, & Hillenkamp, 1985). A laser beam is then introduced which results in a burst of ions. The presence of the matrix refreshes the laser burst, and enhances the isolation of the sample ions.
Protein and peptide analyses are the most common applications of MALDI, with the most number of technical developments in the past years (Hillenkamp & Katalinic, 2007). MALDI is the leading application for proteomics profiling and imaging. Other analytes studied are nucleic acids, glycans, lipids, and synthetic polymers. Each of these analytes may have limitations in their analysis due to their structural properties, which can interfere with the ionization efficiency. The spatial distribution of drugs, metabolites, and proteins in intact tissues is also made possible with imaging mass spectrometry (Caldwell & Caprioli, 2005). MALDI-MS is therefore a valuable technique that can address a broad range of applications in the biomedical field.
Since MALDI-MS tissue profiling permits the detection of more than a thousand peptides and proteins from many tissue types, it is very useful in detecting disease processes. Tissue profiling and imaging permits the direct acquisition of mass spectra from intact tissues that are either freshly prepared or come from tissue storage banks (Djidja, et al., 2010). Recently, tissue blocks that have been embedded in paraffin were used in protein profiling of several cancers. These studies showed that
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Hi Need To Submit A 2000 Words Essay On The Topic Is Adolescence A Time Of Storm
/in Uncategorized /by developerHi, need to submit a 2000 words essay on the topic Is Adolescence a time of ‘storm and stress’ (individual development module) psychosocial study degree.
The adolescent is confronted with many different changes and is able to adapt to these changes in a constructive fashion, and in a way that results in developmental advance. Considering the large variety of tasks and problems encountered, adolescence is characterized by impressively effective coping in the majority of young people, a fact that has been widely neglected.
Coping is the effort to manage (i.e., master, reduce, minimize) environmental and internal demands and conflicts which tax or exceed a persons resources and this shows that coping is a hypothetical construct that is sufficiently complex to take into account both person-specific and situation-specific aspects. Stressors and social resources are also two important concepts. The significance of coping behavior is evident in resiliency research showing that it is coping that makes the difference in both the adaptational outcome and in research on symptomatology, illustrating that the most reliable predictor for mental health is not so much a lack of symptoms, but the competence with which age-specific developmental tasks are handled (Compas & Hammen 1993)
More than any other developmental period, adolescence has been characterized in the psychological and sociological literatures as fraught with struggles that are both intrapersonal and interpersonal in nature. In the intrapersonal domain, adolescence has been described as a period in which identity formation is a central developmental task. Achieving a sense of personal autonomy and an identity that is separate from the family is of utmost importance. Significant interpersonal tasks during adolescence are thought to include increased involvement with the peer group balanced against continued attachment to the family.
Adolescence is a period in which relationships outside of the family multiply, take on new meanings, and deepen in intensity. These new bonds broaden and enrich the
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Hi Need To Submit A 2000 Words Essay On The Topic La Primavera Or Spring And The
/in Uncategorized /by developerHi, need to submit a 2000 words essay on the topic La Primavera (or Spring) and The Tree of Death and Life.
Botticelli’s masterwork implies poetic allegory embracing the theme of love and a certain philosophical meaning as it depicts the Garden of Hesperides, which I will dwell on in more detail below. The plot of the painting is being developed from right to left. There is an ominous figure with wings and swollen cheeks dressed in a greenish flowing cloak in the upper right corner of the painting. His name is Zephyrus and he is a symbol of the wind. One can see how the trunks of trees bent influenced by the flight of this mysterious figure. A scared nymph named Chloris, to the left from Zephyrus, tries to run away touching another woman on the left as if asking her for protection. But the woman neglects the nymph, who tries hard to flee from the wind on the right from her, and is walking to the bottom of the painting. It must be noted that this woman in a long dress embroidered with flowers, with a thin face that registers no emotion and a wreath on the golden hair is depicted in the aforementioned Ovid’s poem Fasti that Botticelli was inspired with when creating his masterpiece. In Ovid’s poem she is referred to as Spring but, as a matter of fact, this woman might as well be the goddess of flowers and plants, Flora. In the Botticelli’s painting she had a dress embroidered with red and blue cornflowers, symbols of amiability and good nature, and scattered roses, which was the usual thing for rich Florentine weddings. The wreath around her neck is made of strawberries that symbolize tenderness, chamomile.
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Hi Need To Submit A 2000 Words Essay On The Topic Leadership Plan For Hurricane
/in Uncategorized /by developerHi, need to submit a 2000 words essay on the topic Leadership Plan for Hurricane Andrew.
This paper will come up with a leadership plan for addressing the case study outlying Hurricane Andrew and the challenge it brought to the Florida communities.
Hurricane Andrew was a catastrophic cyclone that was, at that time, the worst hurricane in the history of the United States. The fourth tropical hurricane, initially named the storm, and then the 1992 Atlantic hurricane season, Hurricane Andrew originated from a tropical wave over the Atlantic on August 16, 1992, from West Africa. At first, strong wind shear stopped much escalation (Waugh, 2000). A decline in shear the next day allowed the depression to strengthen turning into Tropical Storm Andrew by 1200 UTC. This was on 17th August (Waugh, 2000). Nevertheless, increased wind shear on 18th August diminished convection linked to the storm. In the next two days, wind gradient drastically reduced, and Andrew became a minimal cyclone on August 22. Thereafter, the hurricane turned westward under the power of a high-pressure system and began quickly to strengthen later that day. Shortly before moving through the Bahamas, Hurricane Andrew strengthened into a Category 5 cyclone on 23rd August. The cyclone weakened vaguely over the Bahamas to a Category 4 cyclone, but shortly re-intensified into a Category 5 cyclone on 24th August before making landfall on Elliott Key, and afterwards in Homestead, Florida. The hurricane’s effects were mostly felt in Florida as it resulted in mass human displacement and property damage. Assessors estimated that the damages from the hurricane resulted in almost $20billion making it the most devastating and expensive cyclone ever to hit the U.S. (Waugh, 2000).
Hence, it was vital to make sure that people recovered fully from the hurricane’s effects and were restored to their previous lives. The United States had a Federal Response Plan (FRP) in place. This was their response arm in case of such calamities. However, the
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Hi Need To Submit A 2000 Words Essay On The Topic Lost In Digital Wonderland Fin
/in Uncategorized /by developerHi, need to submit a 2000 words essay on the topic Lost in Digital Wonderland Finding a Path in the Global Knowledge Society.
Such a transformation took place mainly due to the increased need for innovation. As the rate of trade dealings is enhancing in the entire globe, the ratio of competitiveness is also expanding. However, in order to cope up with such type of competitive needs or demands of the citizens, the labours or the producers need to develop highly extensive abilities and knowledge (Max-Planck institute for history and science, n. d.). Such type of demanding skills and knowledge might be attained only through varied types of learning programs, training programs such as on-the –job, household trainings etc. Only then, the employees or the individual might easily cope up with the risks or changing demands of the citizens in global economy. By doing so, the producer or the employees might position itself as a successful individual or organization in this global knowledge society as compared to others.
Knowledge is described as a resource that helps to generate values and creativity. In this age of digitalisation, it is the knowledge that helps in enhancement of the dealings of trade leading to economic growth and development of a nation in the entire globe among others. Other than this, it is the knowledge that helps an individual entrepreneur to cope up with the changing requirements of the citizens by presenting inventive products. However, the inventive products are mainly developed with the help of new ideas and suggestions as well as advanced technologies (Huws, 2005). Only then, the employer or the employees in this revolutionary age might create a specific position for itself. Thus, it might be stated that knowledge is the prime requirement of an employee or employer to tackle global competitiveness.
Knowledge society is recognised as a place, where all sorts of activities or actions take place with the help of both tacit and codified knowledge. Both tacit as well as codified knowledge is utilised in order to perform the knowledge works in this information age
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Hi Need To Submit A 2000 Words Essay On The Topic Maldi Technique Flow Cytometry
/in Uncategorized /by developerHi, need to submit a 2000 words essay on the topic MALDI technique & FLOW CYTOMETRY technique.
(2) ionization of sample components to produce ions, (3) separation of ions by electromagnetic field to their mass-to-charge ratios, (4) detection of ion signals and, (5) processing of the signals into mass spectra.
The ionization of the analytes is a crucial step in mass spectrometry. Two methods are used: electron spray ionization and matrix-assisted laser desorption ionization (MALDI) (Figure 1). In MALDI, the analyte is mixed with matrix, molecules that have strong absorbance at the laser wavelength, and placed onto a metal plate (Karas, Bachmann, & Hillenkamp, 1985). A laser beam is then introduced which results in a burst of ions. The presence of the matrix refreshes the laser burst, and enhances the isolation of the sample ions.
Protein and peptide analyses are the most common applications of MALDI, with the most number of technical developments in the past years (Hillenkamp & Katalinic, 2007). MALDI is the leading application for proteomics profiling and imaging. Other analytes studied are nucleic acids, glycans, lipids, and synthetic polymers. Each of these analytes may have limitations in their analysis due to their structural properties, which can interfere with the ionization efficiency. The spatial distribution of drugs, metabolites, and proteins in intact tissues is also made possible with imaging mass spectrometry (Caldwell & Caprioli, 2005). MALDI-MS is therefore a valuable technique that can address a broad range of applications in the biomedical field.
Since MALDI-MS tissue profiling permits the detection of more than a thousand peptides and proteins from many tissue types, it is very useful in detecting disease processes. Tissue profiling and imaging permits the direct acquisition of mass spectra from intact tissues that are either freshly prepared or come from tissue storage banks (Djidja, et al., 2010). Recently, tissue blocks that have been embedded in paraffin were used in protein profiling of several cancers. These studies showed that
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Hi Need To Submit A 2000 Words Essay On The Topic Marriage In Crisis After This
/in Uncategorized /by developerHi, need to submit a 2000 words essay on the topic Marriage in Crisis.
After this, the next step is to analyze the families based on the multi-generational transmission concept. Since it is generally accepted that those who were abused generally repeat the cycle by becoming abusers, this is probably the case with Luis. By allowing him to understand this and reviewing his own feelings as a child, he can develop empathy for what he is putting his children through. Furthermore, some of the issues Jessica is causing also need to be addressed. Jessica’s relationship is unstable, as Bowen argued that many two-person relationships are. Therefore, to bring about stability, she tends to involve her older son. This is unhealthy for her son since he is too young to understand fully the repercussions of what is going on. Therefore, this situation needs to be addressed, and Jessica needs to be taught alternate methods for handling her issues.
 .Genograms cam is a very helpful tool in a number of situations. In the situation of Jessica and Luis, it can provide a helpful visual chart that may help to make them more aware of their bad situation. Jessica and Luis are undergoing many relationship issues, and genograms can be helpful in providing awareness in issues like this. The genogram itself “is a visual representation of an individuals family of origin and is similar in concept to a family tree” (Daughhetee, 2001, p. 21). It has been used for many decades in family therapy and was initially created by Murray Bowen. The genogram allows the counselor and the family to take a look at the relationships, roles, patterns, and functions that are common within the family. This can, therefore, allow the counselor to gather important data, cover levels of functioning, create a hypothesis, and increase the awareness of the family regarding the emotional processes and repercussions their family structure may be undergoing (Daughhetee, 2001).
 .
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Hi Need To Submit A 2000 Words Essay On The Topic Merchandising Analysis Report
/in Uncategorized /by developerHi, need to submit a 2000 words essay on the topic Merchandising Analysis Report.
Proper merchandising can lead to selling of quality products by the employees to the customers along with facilitating them to deliver better customer service (Jackson & Shaw, 2000). Poor merchandising on the part of an organisation can make it quite tough for the sales staff in a retail store to sell products to the customers, which would effect on the productivity and the profitability of the stores by a greater extent. By incorporating well-prepared merchandising initiatives, it is possible to convert potential consumers into real consumers of a company. The process of merchandising provides a competitive advantage to a company which can further be significant in ensuring its success or failure as retail business outlets in a sector such as fashion based designer products (Queensland Government, n.d.).
Fashion merchandising entails an amalgamation of different procedures which a fresh fashion product is required to go through to be accessible in sufficient quantities to the customer after it is prepared by the designer. Fashion merchandising encompasses various pertinent aspects such as target market and price. A key constituent of fashion merchandising is observed to be selling (Frings, 2002).
The process of cross selling evolves when customers purchase one of the two products of a company. Through the process it is also possible to recognize the customers’ who are purchasing a single product out of the two products which also results in reduction of total campaigning costs, enhancement in response rates along with facilitating to provide concentrated efforts to increase sales. The consequences of cross selling campaigns include the identification of similarities in a range of products and recognising or estimating the previous as well as future trends of selling (MicroStrategy Inc., 2013).
Offering discounts is also one of the merchandising strategies to attract shoppers to arrive at the stores, which
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Hi Need To Submit A 2000 Words Essay On The Topic Multiculturalism In Contempora
/in Uncategorized /by developerHi, need to submit a 2000 words essay on the topic Multiculturalism in contemporary english literature(how to do a research for this topic).
multicultural education, or multicultural menu.” As pertains to contemporary English literature, contemporary multicultural English literature would be that body of written work by authors whose work comes to fruition during the twentieth century, incorporating themes and characters of a cultural diversity. such as the works of Salman Rushdie, wherein Rushdie’s work, as Deepika Bahri (2004) suggests, is reflects a body of work that represents “’hybrid temporalities of ‘of colonialism.” Rushdie’s work, as Bahri suggests, falls within the “paleocolonial” era. or those post-colonial writers who by time and space in a post-colonial empire, whose work has not been the subject of post-colonial resentment and suppression.1 While there indeed exists a variety of contemporary English literary works from which to select that include a multicultural diversity by way of author and theme, with the exception of a limited, albeit growing, body of works, they tend to be works portraying the English protagonist in a foreign setting. The goal of this paper became one of searching the existing body of contemporary literature for a work of literature reflecting a multicultural setting, written by author of other than a British born English heritage, and whose story presents a protagonist as hero, or heroine of that story in a multicultural setting and of a multicultural heritage. To that end, the focus rested on the works of author Salman Rushdie, whose body of work emanated from lands other than England, and brought to the reader the opportunity to both discover the essence of a rich multicultural environment and tradition through interesting multicultural characters. Narrowing down the selection within Rushdie’s body of work to the multicultural work encompassing the goals previously discussed, the focus of this paper is Rushdie’s The Moor’s Last Sigh (1997).2
The selection of support materials for inclusion in this paper were made on the basis of
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Hi Need To Submit A 2000 Words Essay On The Topic Neuroscience The Center 4 Tile
/in Uncategorized /by developerHi, need to submit a 2000 words essay on the topic Neuroscience.
the center 4 tiles), the amount of time a rat spent in the center of the field (i.e. the center 4 tiles), and the number of tile crossings all indicated that rats treated with LiCl had indeed reduced risk taking, locomotor activity and exploratory behavior when undergoing the open-field test compared to sodium chloride (NaCl) treated control rats. Note, the tendency of the rat to cling and spend much time on the walls of the test apparatus indicates a high level of anxiety or fear. On the contrary, a less fearful rat portrays the tendency of greater exploration within the experiment apparatus. As such, the rats that had been injected with LiCl spent much time on the walls of the apparatus – a sign of increased fear and high level of anxiety. The results obtained in this test were in agreement with other studies previously conducted.
Laboratory observations have suggested an interaction between lithium chloride (LiCl) and exploratory behavior of rats. Studies have confirmed that LiCl administration tends to suppress locomotor activity in rats (Johnson et al., 1972). An open field apparatus was used to observe the exploratory behavior and the movement of the rats one hour after the injection of LiCl. The purpose of the present study was to examine the effects of LiCl on risk taking, exploratory behavior, and locomotor activity of rats.
The behavioral pharmacology test conducted was aimed at seeing the effectiveness of Lithium chloride on the various specimens used and their response. The experiment used Lithium Chloride as the main ingredient and sodium chloride as the control solution. The main objective of the experiment was an observation of the effects of taking against not taking Lithium chloride and on the other hand taking sodium chloride. We used an Open Field Apparatus from whence we could observe the behavior of the rat immediately after the injection with the drug.
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Hi Need To Submit A 2000 Words Essay On The Topic Nursing Assessment Download Fi
/in Uncategorized /by developerHi, need to submit a 2000 words essay on the topic Nursing Assessment.
Download file to see previous pages…
Firstly, the patient should be asked about the details of the shortness of breath by defining the symptoms as well as finding how long the patient has been in that condition. Some of the main variables to identify in the symptoms include onset time, location, severity, duration, radiation and quality. Secondly, there would be the establishment of the history of symptom obtained through talking to the patient about the frequency of short breath. This may uncover the pattern in the symptom presentation and may help determine any respiratory problem if the patient reports regular physical exertion. Thirdly, there will be discussion on pre-existing patient’s condition in order to obtain a comprehensive medical history about the patient. Any information on hospitalizations of the patient enhances determination of factors that can result or contribute to breathing difficulties. At this point, the medications should be discussed since some drugs may result side effects, including the shortness of breath. The other questions will involve the eating habits. For coughing, the patient should be asked the frequency of coughing and the duration of coughing. The information on the blood or mucus and the color can necessitate getting of some information. The patient will then be asked how the obesity condition affects the life quality like disrupted routines (Beaman, 2011). Physical examination may involve first determining the BMI and examining the body clearly. Secondly, the lungs must be examined. This involved determination of the body weight, temperature and the body mass index (BMI) that entails measuring the height in order to enhance the determination of the effects of weight of the health condition. Also, this would involve listening to lungs and heart beat using stethoscope. Checking of the signs of blood backing up in the neck veins may facilitate determination of cor pulmonale disorder of the heart. Then there should be a full cardiac examination that involves listening to the six sections of the heart with the diaphragm and the bell of the stethoscope. This may reveal underlying cardiac condition like murmur which results from incompetent heart valve. Thereafter, the respiratory rate of the patient must be measured to enhance quantification of the shortness of breath. The normal respiratory rate for an adult ranges from 12-18 breaths per minute and the extent of deviation from the normal rate indicates the severity of the patient’s breathing condition (Utian, 2004). Pressing the abdomen enhances determination of abdominal palpation. The lips and fingers must be assessed for cyanosis while fingers must be checked for any clubbing. Physical examination might be a little painful, especially the abdominal palpation. Prioritized health history and physical assessment enhances diagnosis, and must be performed on a regular basis as they may reveal risk factors that suggest increased risk. The physical risks will enhance assessment of severity of conditions such as the inability of completing full sentences without having to take a breath (Beaman, 2011). A prioritized history and assessment of the heart enhances the exclusion of heart diseases associated or, which may result into a similar situation. Based on the assessments above, two interventions that I would prioritize include heart and lung interventions.
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